Comparative evaluation of efficiency of rapid urease test helpyl for diagnostics of Helicobacter Pylori in stomach and duodenum

Konorev M.R., Matveenko M.E., Krylov U.V., Komleva E.N., Kovalenko T.V.

To determine the efficiency of rapid urease test HELPYL for diagnostics of H. pylori (Hp) in gastric and duodenal mucous at patients with dyspepsia syndrome. Diagnostic, cross-section research (n=107) with application of Latin square (table 2х2) for comparison of rapid urease test HELPYL (АМА, Russia) and rapid urease test Jatrox-H.p.-Test (Rohm Pharma, Germany) is carried out. Examination of H.pylori infection in gastric and duodenal mucous by Giemsa used as comparison method. Regions of gastric metaplasia of duodenum were confirmed by periodic acid-Schiff and alcian blue staining (pH 1.0; 2.5). The estimation of efficiency was carried out at the same patients. Results of estimation of efficiency rapid urease test HELPYL (gastric and duodenal mucous respectively): sensitivity (Se) – 0.97; 0.91; specificity (Sp) – 0.98; 0.98; prevalence (P) – 0.54; 0.20; test accuracy (TA) – 0.97; 0.96; negative predictive value (-PV) – 0.96; 0.98; positive predictive value (+PV) – 0.98; 0.91; positive likelihood ratio (LR+) – 48.3; 39.3; negative likelihood ratio (LR-) – 0.03; 0.1. Results of estimation of efficiency rapid urease test Jatrox-H.p.-Test (gastric and duodenal mucous respectively): Se – 0.98; 0.95; Sp – 0.98; 0.99; P – 0.54; 0.20; TA – 0.98; 0.98; -PV – 0.98; 0.99; +PV – 0.98; 0.95; LR+ – 49.0; 79.3; LR- – 0.02; 0.05. HELPYL-Test and Jatrox-H.p.-Test have high clinical efficiency for diagnostics H.pylori in gastric and duodenal mucous. HELPYL-Test in 480 times reduces time of diagnostics of H.pylori in comparison with Jatrox-H.p.-Test and allows to use in further biopsy of gastric and duodenal mucous for morphological research.