Enhanced early detection of blood-contaminating microorganisms with use of yeast extract
Viatkina O.I., Potapnev M.P., Tsˇrkunova Zh.F.
Republican Scientific and Practical Center for Transfusiology and Medical Biotechnologies, Minsk
Belarusian State Medical University, Minsk
The effect of yeast extract (YE) on the growth of microorganisms, including those incubated with peripheral blood, was studied. It has been shown that the growth of E. coli ATCC 11229, P. aeruginosa ATCC 15442 and C. albicans ATCC 10231 is suppressed in the presence of donor blood. It has been established that the ability of peripheral blood to suppress microorganism growth can be overcome by cultivating microorganisms on nutrient media containing YE at the concentration of 10 g/l. Experimental studies have shown that the addition of YE to liquid nutrient media increased the sensitivity of the latter to cultures of P. aeruginosa ATCC 15442 and C. albicans ATCC 10231, as well as clinical isolates of S. aureus and C. albicans. A conclusion was made concerning the ability of YE in concentrations of 1.0% (10 g/l) to improve the nutritional properties of nutrient media (sensitivity and growth rate) during the cultivation of microorganisms.