Clonal spreading of Pseudomonas aeruginosa strains " metallo"beta" lactamases producers in territory of Belarus

Osipov V.A., Tapalski D.V., Sleenova E.Yu., Romanov A.V., Zhavoronok S.V.

19 carbapenem"resistant isolates Pseudomonas aeruginosa from six medical organizations from Gomel, Minsk and Mogilev was investigated. Multiplex real time PCR was used for detection of blaVIM and blaIMP MBL"genes. PCR"restriction mapping method was used for studying of structure of integrons carrying genes of MBL. Epidemiological marking for MBL"positives isolates of P.aeruginosa was carry out with using of multiple"locus variable number tandem repeat analysis (MLVA) in six VNTR"loci. The cluster analysis of the received MLVA"patterns was performed.
The presence of blaVIM"genes in 19 carbapenem"resistant isolates was detected. Identity of structure of integrons carrying gene of MBL was established for all isolates.
The belonging of 18 from 19 MBL"positives strains to the common clonal complex was detected by results of a MLVA" typing. The clonal spreading of MBL"producing strains of P.aeruginosa in territory of republic was shown.