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International journal of Immunopathology, allergology, infectology.

Phenol-containing allergen diluting fluid induces the release of myeloperoxidase
into the oral fluid

Matsko E.F., Aliakhnovich N.S.

Vitebsk State Medical University, Vitebsk, Belarus

Aim. To evaluate the eff ect of diluting fl uid for allergens on oral fl uid myeloperoxidase activity (OFMA) in comparison with saline solution in patients with allergic pathology and healthy people.
Materials and methods. 21 patients with established chronic allergic disease (study group), 22 clinically healthy volunteers with a history of allergic reactions (risk group), and 22 without burdened allergic history (control group) underwent an oral provocative test (OPT) with 0.1 ml of non-infectious allergens diluting fl uid in concentration 1:10 and 1:100. 15 participants (8 patients and 7 healthy volunteers) underwent a test with 0.9% saline solution for control. Oral fl uid was collected on an empty stomach and 40 minutes aft er provocation. OFMA was evaluated using a substrate-chromogenic mixture in all samples. Prick-test was made to all participants with saline solution and diluting fl uid (1:10, 1:100).
Results. In 29% (6 out of 21) patients, there was an increase OFMA level after test with diluting fluid (min.+43.2%,max.+67.0%), M=19.1[7.5;30.8] which was signifi cant higher than in the control group M=4.04[-0.5;8.6], (p=0.01). Among the risk group, a signifi cant increase OFMA level was found in 23% (5 of 22) participants (min.+42,8%; max.+49,0), M=12.7[3.5;21.8]. Th e OFMA level growth aft erprovocation with diluting fluid in the study group (p1 <0.0001),risk group (p2 =0.0008) and control group (p3 = 0.001) were significantly higher than with saline solution. The test with saline solution decreases OFMA level in 88% (7 of 8) of allergic patients and 71% (5 of 7) of healthy volunteers. A skin prick test with a diluting fl uid was positive in one case among the participants of the risk group, with saline solution - negative in all cases in the study.
Conclusions. OFMA level increases in 29% (6 out of 21) patients with chronic allergic diseases (p=0,01) and 23% (5 out of 22) people with an allergic history (p=0,08) after OPT with a phenol-containing allergen diluting fl uid. In this case, saline solution causes a decrease OFMA level in 88% people with allergic pathology and in 71% healthy people. OFMA level increases aft er provocation with a diluting fluid among patients with allergic pathology (p1<0.0001), people with a history of allergies (p2=0.0008) and healthy people (p3=0.001) is signifi cantly higher than with saline solution. When conducting provocative tests, it is recommended to dilute allergens with saline solution, since it doesn't contain phenol and doesnt infl uence on OFMA level.


Diluting fl uid, oral provocative test, oral fluid myeloperoxidase activity, skin prick test, phenol.

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Matsko E.F., Aliakhnovich N.S. Immunopathology, allergology, infectology 2019; 4:82-88. DOI: 10.14427/jipai.2019.4.82