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International journal of Immunopathology, allergology, infectology.

Genetic diversity of Acinetobacter baumannii strains producing carbapenemases in Belarus: the role of "international high-risk clones" in the spread of resistance to carbapenems

Shek E.A.,Tapalski D.V., Skleenova E.Yu., Sukhorukova M.V., Karpov I.A., Edelstein M.V.

1 Institute of Antimicrobial Chemotherapy, Smolensk, Russia
2 Gomel State Medical University, Gomel, Belarus
3 Belorussian State Medical University, Minsk, Belarus

The rapid spread of extensively drug-resistant carbapenemase-producing A. baumannii strains has been noted in various regions of the world. The objective of the study is to assess the presence of various acquired carbapenemases among carbapenem-resistant A.baumannii clinical isolates collected in Belarus as well as the genetic diversity of the isolates and their attribution to the international high-risk clones
Ninety-one carbapenem-resistant A.baumannii clinical isolates were included in the study. Genes of acquired carbapenemases were detected by real-time PCR. Genetic diversity of isolates was assessed by SNP-typing, based on the analysis of single nucleotide polymorphisms (SNPs) in ten chromosomal loci. blaOXA-23-like, blaOXA-40-like, and a combination of blaOXA-23-like and blaOXA-40-like genes were detected in 4.4%, 93.4%, and 2.2% of the isolates, respectively. Using the SNP-typing, the A.baumannii isolates were assigned to 13 different genotypes belonging to 5 clonal complexes. 42.9% of the isolates were referred to international clonal complex CC92OXF/CC2PAS and 25.3% to CC109OXF/CC1PAS. The vertical distribution of OXA-carbapenemase genes as part of "international high-risk clones" is shown, as well as the possibility of horizontal gene exchange between the representatives from various clonal groups.


Acinetobacter baumannii, antimicrobial resistance.




Shek E.A.,Tapalski D.V., Skleenova E.Yu., Sukhorukova M.V., Karpov I.A., Edelstein M.V. Immunopathology, allergology, infectology 2018; 2:59-64. DOI: 10.14427/jipai.2018.2.59