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International journal of Immunopathology, allergology, infectology.

Allocation of a tryptase in stomatic liquid and activation of basophiles at an intolerance of stomatologic materials

Karpuk I. Yu.

Vitebsk State Medical University, Vitebsk, Belarus

The goal of the study was to determine the levels of mast cell tryptase (MCT) in whole saliva and IgE antibodies in blood in patients with intolerance to dental prosthetic materials (intolerance to dental materials IDM) before and after removal of prosthetic constructions. We have conducted an examination of the patients suffering from the symptoms of IDM, who were divided into 2 groups depending on the time span between the end of prosthetic treatment and the emergence of pathological symptoms: group 1 (n=19) from 1 to 14 days (emergence of symptoms took place immediately after treatment); group 2 (n=18) from 6 months to 5 years, group 3 (n=16) controls no complaints of IDM. Whole saliva (WS) samples were collected before removal of prosthetic constructions and 1 month after. In group 1 patients we could detect salivary MCT in 16 (84,2%) subjects before removal of prosthetic constructions, and 1 month after MCT in whole saliva was not found (p<0,001). MCT in whole saliva of control group patients was not detected both before and after removal of prosthetic constructions. Thus, mast cell tryptase in whole saliva is a diagnostic marker for intolerance to dental materials. In group 1 patients we detected IgE antibodies to Ni-HSA in 78,9% of patients, IgE antibodies to Cr-HSA in 68,4% of patients and IgE to CoHSA in 52,6% of patients. Salivary MCT levels were in strong correlation with IgE levels to Ni-HSA (RSpearman=0,9; p<0,05) and in moderate correlation with Cr-HSA (RSpearman=0,7; p<0,05). The obtained data suggest the prevalence of immediate type reaction to dental materials. Expressed local increase of MCT level is an important diagnostic marker of local inflammatory process initiation. MCT in whole saliva was found only in 3 (16,7%) patients from group 2, the same 3 patients had IgE antibodies to metal ions in blood serum, which indicates IgE-dependent type of reaction. The rest of the patients from group 2, most likely, have a different type of allergic reaction,
e. g. delayed or granulocyte-mediated. For reliable diagnosis of allergy to the components of dental materials it is reasonable to measure salivary MCT before and after removal of prosthetic constructions. Salivary MCT level monitoring allows to assess the role of prosthetic constructions for the emergence of IDM and need for their replacement.


Allergy, IgE-antibodies, tryptase, mast cells, whole saliva,
dental materials

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Karpuk I. Yu. Immunopathology, allergology, infectology 2017; 3:22-29. DOI: 10.14427/jipai.2017.3.22